![]() Therefore, western blot is also known as an immunoblot. Western blotting is a method of detecting a specific protein from a protein mixture by the use of labeled antibody. ![]() Northern blotting is an important tool in detection and quantification of hybridized mRNA, studying RNA degradation, evaluation of RNA half-life, detection of RNA splicing, studying gene expression, etc.įigure 02: Northern Blotting What is Western Blotting? Hybridized fragments are detected by an autoradiograph.The probe is incubated with the membrane to bind with a specific sequence.The membrane is treated with a labeled probe prepared from cDNA or RNA ( the probe is complementary to a specific sequence in the sample).RNA is transferred from the gel onto a blotting membrane and fixed.RNA is extracted from the sample and separated by gel electrophoresis.Northern blotting technique is performed as follows. ![]() However, this technique is also performed via gel electrophoresis, blotting, and hybridization with specific labeled probes and detection. It differs from the sourthern and western blotting techniques due to several steps. This technique was developed by Alwine, Kemp, and Stark in 1979. Northern blotting is a method designed to detect a specific RNA sequence or mRNA sequence from a sample to study gene expression. It is useful in RFLP mapping, forensic studies, DNA methylation in gene expression, detection of mutated genes in genetic disorders, DNA fingerprinting, etc. Southern blotting is applied for different aspects of molecular biology.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |